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Neuromics

Primary Antibody · Mouse IgG

Beta-Tubulin

Species
Human, Mouse, Rat, Primate
Applications
ICC, WB, IHC, IF

Description

Tubulins are the major protein components of cytoplasmic microtubules. β-tubulin is regarded as a “house keeping” protein which is generally not altered much in expression as a result of experimental manipulations. As a result antibodies to β-tubulin are widely used as loading controls in western blotting experiments as a standard by which the levels of other proteins may be measured. The antibody is an ideal western blotting standard as it stains a single ~50kDa band on western blots and also produces beautiful IF images of cells in tissue culture. It will also work well in ICC and IHC. Mouse select image at left for larger view.

Immunofluorescence analysis of HeLa cells stained with mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C, dilution 1:2,000, in red.
Immunofluorescence analysis of HeLa cells stained with mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green, and costained with chicken pAb to lamin A/C, dilution 1:2,000, in red.
Western blot analysis of equal amounts of cell line and whole brain lysates using mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green.
Western blot analysis of equal amounts of cell line and whole brain lysates using mouse mAb to β-tubulin, MO22180, dilution 1:5,000 in green.

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For research use only. Not for diagnostic or clinical use.