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Neuromics
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Primary Antibody · Rabbit IgG

Green Fluorescent Protein (GFP)

Species
Not Applicable
Applications
WB, IHC, IF

Description

GFP (green fluorescent protein) is 27kDa protein; which was original isolated from the jellyfish (Aequoria victoria). This antibody was made against a recombinant GFP, from the Aequoria species, referred to as AcGFP. This antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, RA22134, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.
Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, RA22134, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The GFP antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.
Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.
Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP (in green), dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct.

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For research use only. Not for diagnostic or clinical use.