Published with this product (3)

2025Biofabrication
Non-Destructive Luminescence and PET Imaging to Monitor Tissue Microenvironment in Microphysiological Systems During Brain Metastasis Using Dissociated Cerebral Organoids
Catherine Reed-McBain, Rithvik V. Turaga, Seth R.T. Zima, Janmesh Patel, Anderson Weber Faletti Cunha, Jason Mixdorf, Lauren E Wehner, Jonathan W. Engle, Reinier Hernandez, Stevens K Rehen, Helena L Borges, and Jose M Ayuso
doi:10.1088/1758-5090/ade1fb
2015BMC Neuroscience, 201516:76
Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons
Chelsea M. Larabee, Constantin Georgescu, Jonathan D. Wren and Scott M. Plafke
doi:10.1186/s12868-015-0194-y
2012PLoS ONE, 7(4): e35541
Widespread Contribution of Gdf7 Lineage to Cerebellar Cell Types and Implications for Hedgehog-Driven Medulloblastoma Formation
Frances Y. Cheng, Xi Huang, Anuraag Sarangi, Tatiana Ketova, Michael K. Cooper, Ying Litingtung, Chin Chiang
doi:10.1371/journal.pone.0035541

Description

Human GFAP is a 49,749 dalton protein (432 amino acids) expressed in astrocytes. GFAP is an intermediate filament protein and acts as an intracellular structural component of the cytoskeleton. During embryonic and fetal life, GFAP is also expressed by radial glial cells of the CNS. Various mutations of the GFAP gene in humans produce Alexander's disease, one of the leukodystrophies.

Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP.

Mixed neuron-glial cultures stained with Mouse Monoclonal GFAP, and Chicken Polyclonal Neurofilament-NF-L(green). The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes. The blue channel shows the localization of DNA. This antibody also works on formalin fixed paraffin embedded brain tissues. Protocol on Datasheet.

Strip blot of rat spinal cord protein extract stained with GFAP. A prominent band at about 55 kDa corresponds to the major isoform of GFAP.

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