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Neuromics
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Primary Antibody · Mouse IgG

Ankyrin 3

Species
Human, Rat
Applications
ICC, WB, IHC, IF

Description

Ankyrin 3, also known as Ankyrin G, is larger than the related Ankyrin 1 and Ankyrin 2.  Ankyrin 3 is expressed in the axon initial segment and the nodes of Ranvier in the nervous system. Antibodies against Ankyrin 3 thus can be useful in identifying the regions of axon initial segments and nodes of Ranvier. This antibody was made against the C-terminal 398 amino acid of human isotype 1, and works well on human and rat ankyrin 3 on western blots and section material. However, this antibody does not work on mouse extracts and tissues.
Immunofluorescent analysis of cortex section of rat brain stained with mouse antibody against ankyrin 3 (MO22185), dilution 1:2,000 in green and costained with rabbit antibody against NF-L (RA22104) dilution 1:5,000 in red.
Immunofluorescent analysis of cortex section of rat brain stained with mouse antibody against ankyrin 3 (MO22185), dilution 1:2,000 in green and costained with rabbit antibody against NF-L (RA22104) dilution 1:5,000 in red.
Western blot analysis of different tissue and cell lysates using mouse mAb to ankyrin 3, MO22185, dilution 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates.
Western blot analysis of different tissue and cell lysates using mouse mAb to ankyrin 3, MO22185, dilution 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates.

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For research use only. Not for diagnostic or clinical use.