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Neuromics
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Primary Antibody · Rabbit IgG

alpha-II-Spectrin/alpha-Fodrin

Species
Human, Mouse, Rat, Bovine
Applications
ICC, WB, IHC, IF

Description

Spectrin family molecules are important high molecular weight components of the submembranous cytoskeleton of eukaryotic cells. These proteins were isolated originally from lysed red blood cell membrane preparations which were named “ghosts”, which gave rise to the name spectrin. They function as major components of the membraneous cytoskeleton, mediating interactions between integral membrane proteins, actin and many other cellular components. This antibody binds specifically to αII-spectrin, also known as non-erythroid spectrin or fodrin. In the CNS this protein is expressed only in neurons and so the antibody can be used to reveal the submembranous neuronal cytoskeleton in IF, ICC and IHC.

RA22142 was made against the recombinant human protein construct derived from the C-terminus of αII-spectrin comprising the C-terminal 14 spectrin repeats, specifically amino acids 676-2447. This antibody can be used to study αII-spectrin on western blots and to visualize the neuronal plasma membrane cytoskeleton in cells in culture and sectioned material.

Product Highlights:
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to αII-spectrin, RA22142, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat embryos stained with rabbit pAb to αII-spectrin, RA22142, dilution 1:500 in green, and costained with mouse mAb to Ankyrin3 in red. The blue is Hoechst staining of nuclear DNA. The spectrin antibody stains submembranous cytoskeleton of neuronal cells. The Ankyrin3 antibody labels axonal initial segments of neurons.
Western blot analysis of different tissue lysates using rabbit pAb to αII-spectrin, RA22142, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact αII-spectrin.
Western blot analysis of different tissue lysates using rabbit pAb to αII-spectrin, RA22142, dilution 1:2,000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse spinal cord, and [4] cow cortex. The prominent band above 250kDa represents the intact αII-spectrin.

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For research use only. Not for diagnostic or clinical use.