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Neuromics
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Primary Antibody · Chicken IgY

Ankyrin 3/Ankyrin G

Species
Human, Mouse, Rat, Primate, Bovine
Applications
WB, IHC, IF

Description

Ankyrin 3, also known as Ankyrin G, is larger than the related Ankyrin 1 and Ankyrin 2.  Ankyrin 3 is expressed in the axon initial segment and the nodes of Ranvier in the nervous system. Antibodies against Ankyrin 3 thus can be useful in identifying the regions of axon initial segments and nodes of Ranvier. This antibody was made against the C-terminal 398 amino acid of human isotype 1, and works well on human and rat ankyrin 3 on western blots and section material. This segment is expressed by all ankyrin 3 three isotypes and contains the DEATH domain sequence. 
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.
Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.
Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.

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For research use only. Not for diagnostic or clinical use.