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Neuromics
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Primary Antibody · Chicken IgY

MeCP2 (Methyl-CpG Binding Protein 2)

Species
Human, Mouse, Rat, Primate
Applications
ICC, WB, IHC, IF

Description

Methyl-CpG Binding Protein 2 (MeCP2) is widely expressed in tissues and particularly heavily in neurons. It is a nuclear protein which was found to bind methylated cytosines in CpG islands in DNA, but which may also bind hydroxymethyl cytosine residues. This antibody was made against full length recombinant human MeCP2 expressed in and purified from E. coli and can be used to identify neurons transiently expressing high levels of this nuclear protein.

Product Highlights:
Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2, CH22138, dilution 1:2,000 in red and costained with mouse mAb to α-synuclein in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2, CH22138, dilution 1:2,000 in red and costained with mouse mAb to α-synuclein in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
Western blot analysis of tissue and cell lysates using chicken pAb to MeCP2, CH22138, dilution 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein.
Western blot analysis of tissue and cell lysates using chicken pAb to MeCP2, CH22138, dilution 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein.

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For research use only. Not for diagnostic or clinical use.