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Neuromics
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Primary Antibody · Goat IgG

FOX3/NeuN

Species
Human, Mouse, Rat
Applications
ICC, WB, IHC, IF

Description

NeuN has become very widely used as a reliable neuronal marker, apparently binding to neurons in all vertebrates tested. The vast majority of neurons are strongly NeuN positive, and NeuN immunoreactivity has therefore been widely used to identify neurons. The differing protein isoforms of FOX3 result from alternate splicing of two exons which code for an insert close to the C-terminus and a short C-terminal extension. The extension includes a C-terminal proline-tyrosine sequence preceded by hydrophobic amino acids (Φ-PY) which is known to target proteins to the nucleus, apparently accounting for FOX3 being present in both nuclei and cytoplasm in certain neurons.

This antibody was raised against a recombinant human FOX3 construct based only on the N-terminal sequence, not including the RRM domain and C-terminal regions. The N-terminal regions of FOX1, FOX2 and FOX3 are relatively poorly conserved so we were able to obtain antibodies which recognized FOX3 but not FOX2 or FOX1.

Product Highlights:
Immunofluorescent analysis of a section of adult rat hippocampus stained with goat pAb to Fox3/NeuN, GT22106, dilution 1:2,000 in red, costained with mouse monoclonal antibdy to MAP2 in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
Immunofluorescent analysis of a section of adult rat hippocampus stained with goat pAb to Fox3/NeuN, GT22106, dilution 1:2,000 in red, costained with mouse monoclonal antibdy to MAP2 in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies.
Western blot analysis of whole brain lysates using goat pAb to FOX3/NeuN, GT22106, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of FOX3/NeuN.
Western blot analysis of whole brain lysates using goat pAb to FOX3/NeuN, GT22106, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of FOX3/NeuN.

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For research use only. Not for diagnostic or clinical use.