Puromycin 2HCl

Puromycin is an aminonucleoside antibiotic derived from Streptomyces alboniger.  It is a protein synthesis inhibitor that disrupts peptide transfer on ribosomes causing premature chain termination [1]. It is an inhibitor of dipeptidyl-peptidase II and cytosolic alanyl aminopeptidase [2]. Puromycin also inhibits protein transport in mitochondria [3].

References:

1) Azzam and Algranati; (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes  Proc.Nat.Acad.Sci.USA 70 3866

2) Bhutani et al. (2007) Puromycin-sensitive aminopeptidase is the major peptidase responsible for digesting polyglutamine sequences by proteasomes during protein degradation EMBO J. 26 1385

3) Price and Verner (1993)  Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction  Biochim.Biophys.Acta 1150 89