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Neuromics
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Primary Antibody · Mouse IgG

FOX3/NeuN

Cited in 5 papers
Species
Human, Mouse, Rat, Bovine
Applications
WB, IF

Published with this product (5)

Description

Fox3 is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46kDa proteins each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. An alternate name for Fox 3 is hexaribonucleotide binding protein 3, and these proteins are believed to have a role in the regulation of mRNA splicing.

Much interest has focused on Fox3 as this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN antibody has become very widely used as a robust marker of neurons and neuronal stem cells. Fox3 is therefore a protein which has a funciton in RNA splicing and is expressed heavily and specifically in neuronal nuclei.
Rat brain neural cultures stained with FOX3/NeuN (red), and our chicken polyclonal antibody to GFAP (green) and DNA (blue). The FOX3 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. Protocol on Data-sheet.
Rat brain neural cultures stained with FOX3/NeuN (red), and our chicken polyclonal antibody to GFAP (green) and DNA (blue). The FOX3 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes, which are staining with the GFAP antibody, and other kinds of non-neuronal cells. Protocol on Data-sheet.
Cryosections form the spinal cord of a mouse stained with NeuN//Fox3 (Cat#MO22122) Top at 10X Bottom at 20X. Courtesy of Dr Sreyashi Samaddar at College of Staten Island
Cryosections form the spinal cord of a mouse stained with NeuN//Fox3 (Cat#MO22122) Top at 10X Bottom at 20X. Courtesy of Dr Sreyashi Samaddar at College of Staten Island
Blots of crude rat brain extract stained with Fox3/NeuN. It is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa. Protocol on Data-sheet.
Blots of crude rat brain extract stained with Fox3/NeuN. It is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa. Protocol on Data-sheet.

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For research use only. Not for diagnostic or clinical use.